B6-CD45.1
B6-CD45.1
| Strain details | |
|---|---|
| Nomenclature | B6.SJL-Ptprca Pepcb/BoyJ |
| Common name | B6 CD45.1 |
| Synonyms | Pep Boy, Ptprca[a]J, PTP |
| Strain | Congenic, Inbred |
| Coat colour | Black (a/a) |
| Species | Mouse |
| JAX stock number | 002014 |
| Location | Area Oz2 |
| Weekly wean target | 100 males,100 females |
Strain description
- MHC haplotype: H2Kb
- Complement factor: C5 normal
- The B6 CD45.1 strain carries the Ptprca, commonly known as CD45.1 or Ly5.1. Ptprca (protein-tyrosine phosphatase, receptor type c) was the gene symbol designated to the CD45 antigen because of its homology with human placental protein-tyrosine phosphatase 1B. Ptprca is one of a family of protein-tyrosine phosphatase genes involved with the regulation of cell growth.
- This strain is used widely in transplant studies because it carries the differential Ptprcapan leukocyte marker commonly known as CD45.1 or Ly5.1. Wild-type C57BL/6 inbred mice express the Ptprcb (CD45.2 or Ly5.2) allele.
- B6 CD45.1 strain is known to exhibit congenital abnormalities, which include microphthalmia, corneal opacities, anophthalmia, hydrocephalus and portosystemic shunts.
- Created by transferring the Ptprca and Pepcb genes of the inbred strain SJL/J onto the C57BL/6 background through serial backcrossing, and with selection at each generation for Ptprca by serotyping (Shen et al., 1985).
- B6 CD45.1 mice are now one of the most widely used tools in adoptive transfer and mixed-bone-marrow chimera experiments because the transferred cells can be identified by using monoclonal antibodies specific to CD45.2 or CD45.1 (Chisolm et al. 2019).
- Transplant studies, immunology research, cell growth regulation studies.
Past ARC and transfer reports:
Current Ozgene ARC reports (from 01-Jun-2023)
- This strain is housed in Area Oz2/3/5
- Please see Oz2/3/5 health reports on our Biosecurity page
- These mice are sold subject to The Jackson Laboratory’s Conditions of Use and also the Australian Patent Number (2004268530). Information regarding conditions of use is available at: JAX General Terms and Conditions.
- JAX mice are the gold standard for animal model research
- Animals are not allowed to be used for propagation
- Ozgene ARC terms and Conditions
- Mouse images are representative only. Actual phenotypes may vary based on genotype, sex, age, husbandry, health status, and other factors.
- See www.jax.org for more B6 CD45.1strain information.
- Chisolm DA, Cheng W, Colburn SA, Silva-Sanchez A, Meza-Perez S, Randall TD, Weinmann AS. Defining Genetic Variation in Widely Used Congenic and Backcrossed Mouse Models Reveals Varied Regulation of Genes Important for Immune Responses. Immunity. 2019 Jul 16;51(1):155-168.e5. doi: 10.1016/j.immuni.2019.05.006. Epub 2019 Jun 24. PMID: 31248780.
